An evaluation of three methods for fitting the Michaelis-Menten equation to sets of data with a common Michaelis constant but different maximum velocities [proceedings].

and substrate are in direct contact, and the solubility of chlorpromazine therefore appears greater. These previous investigators have fitted data to a normaf Scatchard plot, though there are signs of the plots having been forced through the points at low concentrations. The present results, taken in conjunction with previous ones, indicate a close similarity between chlorpromazine binding to albumin and erythrocytes. Similar co-operative effects have been found for the binding of chlorpromazine and a number of other phenothiazine derivatives to brain protein (A. Rosen & S.-Y. Tham, unpublished work). The lowest drug/protein ratio (0.5) used in this study is higher than that attained physiologically. Previous investigators would therefore have underestimated the amount of free chlorpromazine, which is presumed responsible for therapeutic activity.